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Supplemental Material for Dolan et al., 2023

journal contribution
posted on 2023-09-20, 13:48 authored by Michael DolanMichael Dolan, Nick St. John, Faizan Zaidi, Francis Doyle, Michael Fasullo

File S1 contains the R code for processing barcode counts. File S2 contains the normalized counts for each strain in each screen, as well as the processed differential abundance data. 

Figure S1 shows the interaction of all proteins encoded by the IQ resistance genes identified in the humanized yeast deletion collection under any condition. Figure S2A shows all proteins encoded by the IQ resistance genes in the original yeast deletion collection, and S2B shows the IQ resistance genes appearing twice in screens with the original collection. Figure S3 shows the growth curve data for the humanized double mutants and corresponding humanized single mutants, as well as the humanized WT haploid and diploid controls.

Table S1 contains the list of specific strains used in this study. Table S2 contains the oligos used for high-throughput sequencing and multiplexing. Table S3 contains mutagenicity data from the Ames assay. Table S4 contains the full list of IQ resistance genes identified from the “humanized” yeast deletion collection in either screen. Table S5 contains the list of IQ resistance genes that were independently validated. Table S6 contains GO terms for genes identified in at least two screens of the humanized library. Table S7 contains the full list of IQ resistance genes identified in the original yeast deletion collection. Table S8 contains the GO Terms of IQ resistance genes which confer resistance with or without bioactivation of IQ. 

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Article title

High-Throughput Screening of the Saccharomyces cerevisiae Genome for 2-Amino-3-Methylimidazo [4,5-f] Quinoline Resistance Identifies Colon Cancer-Associated Genes

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