Supplemental Material for McHugh et al., 2020
figureposted on 11.03.2020 by Daniel R. McHugh, Elena Koumis, Paul Jacob, Jennifer Goldfarb, Michelle Schlaubitz-Garcia, Safae Bennani, Paul Regan, Prem Patel, Matthew J. Youngman
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
These data support our functional characterization of DAF-16 during aging in Caenorhabditis elegans. We monitored the survival of daf-16 mutant larvae and adults upon infection with the bacterial pathogen P. aeruginosa to investigate a possible role for DAF-16 in host defense. We also examined age-dependent changes in gene expression, including transcriptional targets of DAF-16, by RNAseq, by qRT-PCR, and by in vivo reporter analysis.