Biernat_Sup_Material.docx (8.59 MB)

Supplemental Material for Biernat et al., 2021

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posted on 08.02.2021, 16:56 by Emily Biernat, Jeena Kinney, Kyle Dunlap, Christian Rizza, Chhabi K. Govind
Figure S1 shows nucleosome profiles generated by MNase-seq, Pol II ChIP-seq profiles and correlation between biological replicates for all datasets. The histograms depicting the MPDF profiles from the two earlier studies (Kubik et al. 2018; Rawal et al. 2018) are also shown. Figure S2 shows RSC enrichment in transcribed CDSs genome-wide. Figure S3 shows 2-dimensional RSC MNase ChIP-seq profiles for replicates. Figure S4 shows genome-browser shots of Pol II and nucleosome occupancies in WT and Sth1 anchor-away cells (Kubik et al. 2018). Table S1 provides names and genotype of the strains used in this study.


Article title

The RSC complex remodels nucleosomes in transcribed coding sequences and promotes transcription in Saccharomyces cerevisiae