## Supplemental Material for Parker et al., 2020

Supplementary Table 1 gives the raw data for the
four week productivity assay of the pure and reciprocal corsses between the O
and B lines. Supplementary Table 2 gives the analyses of variance (ANOVAs) of O
and B line productivity. Supplementary Table 3 shows the genomic regions
containing genetically divergent SNPS. Supplementary Table 4 gives the list of 57 candidate genes tested, including their
FlyBase ID, gene name, gene, symbol, and the Vienna stock number used in the
RNAi assay. Supplementary Table 5 gives the raw lifespan data for *GAL4-c825* × *UAS *RNAi and *GAL4-c825* ×
control F1 flies. Supplementary Table 6 gives the raw lifetime productivity
data for *GAL4-c825* × *UAS *RNAi and *GAL4-c825* × control F1 flies. Supplementary Table 7 gives the raw
weekly productivity data for *GAL4-c825*
× *UAS *RNAi and *GAL4-c825* × control F1 flies. Supplementary Table 8 gives the analyses of variance (ANOVAs) of lifespan
for *GAL4-*c825* × UAS *RNAi and *GAL4-*c825 × control F1 flies.
Supplementary Table 9 gives the analyses of variance (ANOVAs) of lifetime
productivity for *GAL4-*c825* × UAS *RNAi and *GAL4-*c825 ×
control F1 flies. Supplementary Table 10
gives the analyses of variance (ANOVAs) of weekly productivity for *GAL4-*c825*
× UAS *RNAi and *GAL4-*c825 × control F1 flies. Supplementary Table 11
gives the analyses of variance (ANOVAs) of ovary qPCR for *GAL4-*c825* ×
UAS *RNAi and *GAL4-*c825 × control F1 flies. Supplementary Table 12
gives the analyses of variance (ANOVAs) of accessory gland qPCR for *GAL4-*c825*
× UAS *RNAi and *GAL4-*c825 × control F1 flies. Supplementary Table 13
is a summary of genes for which RNAi affects lifespan and/or productivity. Supplementary
Figure 1 shows the results of ovary qPCR for the difference in expression
between *GAL4-*c825* × UAS *RNAi and *GAL4-*c825 × control F1
flies. Supplementary Figure 2 show the results of accessory gland qPCR for the
difference in expression between *GAL4-*c825* × UAS *RNAi and *GAL4-*c825
× control F1 flies.