Supplemental Material for Tramontano et al., 2019

Table S1 contains target names, primer sequences, amplicon lengths and % GC of each amplicon.


Table S2 contains pre-set parameters of sonication of genomic DNA.


Table S3 contains additional tested sonication parameters.


Table S4 contains sequencing statistics for mutant pool 10.


Table S5 contains sequencing statistics for mutant pool 11.


Table S6 contains sequencing statistics for mutant pool 15.


Table S7 contains sequencing statistics for mutant pool 19.


Table S8 contains sequencing statistics for mutant pool 20.


Table S9 contains sequencing statistics for mutant pool 37.


Table S10 contains sequencing statistics for mutant pool 41.


Table S11 contains summary sequencing statistics for 48 barley pools.


Table S12 contains coverage data and calculations for 32 barley amplicons in 48 pools.


Table S13 contains coverage data from a tomato TILLING by Sequencing experiment using non-fragmented amplicons.


Table S14 contains coverage data from cassava sequencing using non-fragmented amplicons.


Table S15 contains frequencies of mutations called with the CAMBa and GATK HaplotypeCaller tools.


Table S16 contains cost estimations for experiments using fragmented and non-fragmented pooled amplicons.


Figure S1 shows Fragment Analyzer profiles of a single amplicon subjected to different default sonication settings.


Figure S2 shows Fragment Analyzer profiles of a single amplicon subjected to different sonication parameters.


Figure S3 shows Fragment Analyzer profiles of a single amplicon subjected to additional sonication parameters.


Figure S4 shows Fragment Analyzer profiles of pooled amplicons before and after sonication.


Figure S5 shows read coverage, base coverage and mapping quality for tomato amplicons not subjected to sonication.


Figure S6 shows insert sizes for 48 barley sequencing pools.


Figure S7 shows principle component analysis of sequencing statistics for 48 barley pools.


Figure S8 contains a graph of mean coverage and amplicon size in 48 barley pools.


Figure S9 contains a graph of mean coverage and percentage of GC in amplicons in 48 barley pools.


Figure S10 contains a graph of percentage of GC and amplicon size in base pairs.