File S1: The sequences for all constructs.

minWT (484 nucleotides)
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagactttattgcagcatcttgaacaatcgtcgcagtttggtaacacgctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattagggg

min∆gt-2 (441 nucleotides)
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagacctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattagggg

extWT (798 nucleotides)
aatataacccaataatttgaagtaactggcaggagcgaggtatccttcctggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagactttattgcagcatcttgaacaatcgtcgcagtttggtaacacgctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggcgcgcaggtccaggcaacgcaattaacggactagcgaactgggttatttttttgcgccgacttagccctgatccgcgagcttaacccgttttgagccgggcagcaggtagttgtgggtggaccccacgatttttttggccaaacctccaagctaacttgcgcaagtggcaagtggccggtttgctggcccaaaagaggaggcactatcccggtcctggtacagttggtacgctgggaatgattatatcatcataataaatgttt

ext∆gt-2 (755 nucleotides)
aatataacccaataatttgaagtaactggcaggagcgaggtatccttcctggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagacctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggcgcgcaggtccaggcaacgcaattaacggactagcgaactgggttatttttttgcgccgacttagccctgatccgcgagcttaacccgttttgagccgggcagcaggtagttgtgggtggaccccacgatttttttggccaaacctccaagctaacttgcgcaagtggcaagtggccggtttgctggcccaaaagaggaggcactatcccggtcctggtacagttggtacgctgggaatgattatatcatcataataaatgttt

ext∆gt-2, ∆gt-4 (755 nucleotides)
AatataacccaataatttgaagtaactggcaggagcgaggtatccttcctggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagacctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggcgcgcaggtccaggcaacgcaattaacggactagcgaactgggttatttttttgcgccgacttagccctgatccgcgagcttaacccgttttgagccgggcagcaggtagttgtgggtggaccccacgatttttttggccaaacctccaagctaacCtgAgATCgtggcaagtggccggtttgctggcccaaaagaggaggcactatcccggtcctggtacagttggtacgctgggaatgattatatcatcataataaatgttt

minWT-sp1 (748 nucleotides)
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagactttattgcagcatcttgaacaatcgtcgcagtttggtaacacgctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGcTGaGTGACTACtcgCtGGTAAcAaTaTCgcTATGGCAcGGTGAAACGCAcaTCGCCAGCGGCACCGCGCgTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGAcCGtGTCtCACTACGTCTGAcCaTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCtgATgTCTAaCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGC

minWT-sp2 (748 nucleotides)
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagactttattgcagcatcttgaacaatcgtcgcagtttggtaacacgctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggACACCGCCGACGGCACGCTGATTGAAGCAGAAGCCTGCGATGTCGGTTcCCGCGtGGTGCGcAgTGAAAATGGTCTGCTGCTGCTGAACGGCAAGCaGTTGCTGATTCCACGCTGATTGAGTCACGAGCATCAgaCTCTGCATCGATGAGCGTTGGATGAGCAGACGATGGTGCAGGATATCCTGCTGATGcAGCAcAACcAaTTTAACGCCGTGCGCcaTTCGCATTATCCGAACCATCCGCTGTGGTACACGCTGTGCGACC

min∆gt-2-sp1
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagacctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGcTGaGTGACTACtcgCtGGTAAcAaTaTCgcTATGGCAcGGTGAAACGCAcaTCGCCAGCGGCACCGCGCgTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGAcCGtGTCtCACTACGTCTGAcCaTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCtgATgTCTAaCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGC

min∆gt-2-sp1+gt-4 (705 nucleotides)
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagacctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattaggggCGCTGTACTGGAGGCTGAAGTTCAGATGTGCGGCGAGcTGaGTGACTACtcgCtGGTAAcAaTaTCgcTATGGCAcGGTGAAACGCAcaTCGCCAGCGGCACCGCGCgTTTCGGCGGTGAAATTATCGATGAGCGTGGTGGTTATGCCGAcCGtacttgcgcaagtCTGAcCaTCGAAAACCCGAAACTGTGGAGCGCCGAAATCCCtgATgTCTAaCGTGCGGTGGTTGAACTGCACACCGCCGACGGCACGCTGATTGAAGC

eveS2_WT
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtcctcgtttcactttcgagttagactttattgcagcatcttgaacaatcgtcgcagtttggtaacacgctgtgccatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatcccgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattagggg

eveS2_∆gt-2
ggttacccggtactgcataacaatggaacccgaaccgtaactgggacagatcgaaaagctggcctggtttctcgctgtgtgtgccgtgttaatccgtttgccatcagcgagattattagtcaattgcagttgcagcgtttcgctttcgtATtcgtttcactttcgagttagacttAActgtgGGatactttcatttagacggaatcgagggaccctggactataatcgcacaacgagaccgggttgcgaagtcagggcattccgccgatctagccatcgccatcttctgcgggcgtttgtttgtttgtttgctgggattagccaagggcttgacttggaatccaatccTgatccctagcccgatcccaatcccaatcccaatcccttgtccttttcattagaaagtcataaaaacacataataatgatgtcgaagggattagggg