setBatchMode("show");
oldTitle=getTitle();
run("32-bit");
run("ROF Denoise", "theta=25");
run("Enhance Contrast...", "saturated=0.3 normalize");
setAutoThreshold("Default dark no-reset");
setOption("BlackBackground", false);
msg = "Adjust threshold sliders (if auto is insufficient), DON\'T CLICK APPLY, click OK here.";
waitForUser("Engulf Cells", msg);
getThreshold(lower1, upper1);
setThreshold(lower1, upper1);
run("Convert to Mask");
run("Fill Holes");
run("Watershed");
run("Invert LUT");
File.append(oldTitle+" : LowerThreshold= "+lower1+" UpperThreshold = "+upper1, getDirectory("image")+"Maps"+File.separator+"ThresholdingInfo.txt");
setBatchMode("hide");