FastQCFastQC Report
Mon 27 Nov 2017
LNN_combine_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename LNN_combine_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 21842230
Filtered Sequences 0
Sequence length 150
%GC 47

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[WARN] Overrepresented sequences

Sequence Count Percentage Possible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC 53322 0.2441234251264637 TruSeq Adapter, Index 7 (100% over 50bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35817 0.1639805093161275 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 12086255 3.1633263 6.1510224 2
GGGAA 5356325 1.7875243 6.13795 1
GGAAA 5487915 1.6005515 5.0014977 1
CGGGG 2790885 1.2171719 5.349396 1
GCGGG 2042935 0.890973 5.490026 1
Produced by FastQC (version 0.10.1)