Supplemental Material for Bandyopadhyay et al., 2021

online resource

posted on 2021-05-11, 14:48 authored by Saptaparni Bandyopadhyay, Joseph Douglass, Sebastian Kapell, Nazimuddin Khan, Fabiana Feitosa-Suntheimer, Jenny A. Klein, Jasmine Temple, Jayce Brown-Culbertson, Alexander H. Tavares, Mohsan Saeed, Nelson C. Lau

BDetal_Bl3sso_SuppLegendsText_20210416.docx
FigureS1_TransgeneDirectionPCRAmplicons_20210416.pdf
FigureS2_PlasmidClasperLabeling_20210415.pdf
FigureS3_AdditionalHDRAssayResults_20210416.pdf
FigureS4_LongSSDnaMiniCircles_20210416.pdf
FigureS5_1stL3SSOtemplates_20210416.pdf
FigureS6_MosquitoAag2CellsandAce2ISG56_20210415.pdf
TableS1_PrimerOligonucleotides_List_20210416.xlsx

History

Article title

DNA templates with blocked long 3' end single-stranded overhangs (BL3SSO) promote bona fide Cas9-stimulated homology-directed repair of long transgenes into endogenous gene loci

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G3: Genes|Genomes|Genetics

Keywords

Cas9 HDR gene tagging BL3SSO Interferon Stimulated Genes Animal Cell and Molecular Biology Genetics Molecular Biology

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CC BY 4.0