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Supplemental Material for Balachandra and Amodeo, 2024
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posted on 2024-11-14, 15:05 authored by Shruthi Balachandra, Amanda A. Amodeo
Bellymount-Pulsed Tracking (PT) is a novel tool that allows continuous, non-invasive live imaging of Drosophila abdominal structures for up to 16 hours-. Bellymount-PT improves upon the existing Bellymount technique by adding pulsed anesthesia with periods of feeding that support the long-term survival of flies during imaging. Using Bellymount-PT we measure key events of oogenesis including egg chamber growth, yolk uptake, and transfer of specific proteins to the oocyte during nurse cell dumping with high spatiotemporal precision within the abdomen of a live female.
