Supplemental Materials for Kramer et al., 2021

Figures described in Transcriptional Profile and Chromatin Accessibility in Zebrafish Melanocytes and Melanoma Tumors. Figure 1 details the experimental design and quality of RNA-seq and ATAC-seq data. Figure 2 summarizes findings from RNA-seq of melanocytes and melanoma cells. Figure 3 summarizes findings from ATAC-seq of melanocytes and melanoma cells. Figure 4 integrates the RNA-seq and ATAC-seq data sets to assess the relationship between gene expression and accessibility. Supplemental Figure 1 visualizes the mCherry melanocytes in skin with scales intact and removed. Supplemental Figure 2 shows the gating used for FACS, as well as H&E staining of fish with and without tumors. Supplemental Figure 3 evaluates gene expression between melanocytes and melanoma cells. Supplemental Figure 4 evaluates differential accessibility between melanocytes and melanoma cells.