Supplemental Material for Thomson et al., 2021
figureposted on 2021-04-05, 16:53 authored by Gregory J. Thomson, Pallavi Kakade, Matthew P. Hirakawa, Iuliana V. Ene, Richard J. Bennett
These are the supplemental figures and excel data files for Thomson et al., Adaptation to the dietary sugar D-tagatose via genome instability in polyploid Candida albicans cells.
Figure S1 shows C. albicans tetraploid RBY18 cells spotted onto SCD or 2-DOG media following growth in different carbon sources in Biolog PM1 or PM2 plates.
Figure S2 shows genome coverage and heterozygosity plots for diploid, tetraploid and D-tagatose-cultured progeny (Tag1-Tag12 strains).
Figure S3 shows allelic frequency analysis for the genomes of control strains (diploid SC5314 and tetraploid RBY18) and for D-tagatose-cultured progeny (Tag1-Tag12 strains).
Figure S4 shows phenotypic analysis of diploid (SC5314), tetraploid (RBY18) and D-tagatose-cultured progeny (Tag1-Tag12). This includes growth rates and cell wall stress assays with Congo Red.
Table S1 shows a list of carbon sources present in phenotype microarray plates PM01 and PM02A.
Table S2 shows oligonucleotides used in this study.