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RamsakEtal2020-G3-Supplemental material.pdf (1.43 MB)
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Supplemental Material for Ramšak, et al, 2020

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posted on 2020-11-13, 15:36 authored by Barbara Ramšak, Jessica Markau, Tobias Pazen, Tim A. Dahlmann, Sven Krappmann, Ulrich Kück

File S1 contains complete protocol for protein synthesis in E. coli. File S2 contains detailed protocol for high-yield purification of the recombinant AfMAT1-1-178-235. File S3 contains protocol for protein quantification and western blotting. Table S1 contains list of oligonucleotides used in this study. Table S2 contains list of plasmids used in this study. Table S3 contains theoretical molecular weights of A. fumigatus truncated MAT1-1-1 proteins. Figure S1 contains multiple alignments of TomA orthologs. Figure S2 contains schematic diagram of the pGEX-4T-1-TEV bacterial expression vector. Figure S3 contains distribution of rare and highly rare codons in the coding sequence of AfMAT1-1-1. Figure S4 contains SDS-PAGE analysis of full-length GST-AfMAT1-1-1 and shortened variant GST AfMAT1 1 11-197 expression in E. coli. Figure S5 contains multiple sequence alignment of selected alpha box domains from representative species of Ascomycota. Figure S6 contains EMSA of binding of various shortened variants of AfMAT1-1-1 to the tom1-2 and tomA-1 dsDNA probes. Figure S7 contains size-exclusion chromatography (SEC) elution profile of AfMAT1 1 178-235.


Article title

The master regulator MAT1-1-1 of fungal mating binds to its targets via a conserved motif in the human pathogen Aspergillus fumigatus

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