Supplemental Material for Li, Vavrik, and Danna, 2020

Figure S1 shows the sgRNA expression cassettes DNA sequence used for targeting genes. This expression cassettes are contained in the T-DNA randomly inserted in the Arabidopsis genome.

Figure S2 shows Cas9 expression in somatic tissue (leaf) of four randomly chosen T1 plants obtained from independent transformation events. Differences in expression are attributed to the chromatin context where the T-DNA was inserted in each independent T1 plant.

Figure S3 shows sgRNA secondary structure predictions for sgRNA used in this study.

Table S1 shows gene-specific 20mer crRNA DNA sequences and predicted efficiency parameters obtained with the CRISPR analysis tools available at MIT (