Supplemental Material for Li, 2019

Figure S1 describes generation of homozygous ΔpyrE::blm (A) and ΔpyrE::kat (B) mutants in T. thermophiles HB27.

Figure S2 is a representative image of dividing T. thermophilus HB27 cells in which septa are not formed at mid-cell positions.

Figure S3 describes generation of homozygous single gene deletion mutant ΔmreB::kat (B) and double gene deletion mutant ΔmreB::kat/ΔparA::blm (C) in T. thermophiles HB27.

Figure S4 describes the allele segregation kinetics of the heterozygous T. thermophiles strain HL01 grown in selection-free liquid medium with and without addition of EDTA.