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Sfig1 Joshi et al.pdf (24.26 MB)

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Table S1 Paper primer List.xlsx (15.81 kB)

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Table S2 Paper Strain List.xlsx (16.29 kB)

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Table S3 junction p-values 1Rdg.xlsx (9.78 kB)

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Table S4 ade6 p-values 1Rdg.xlsx (9.68 kB)

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Table S5 CCE coordinates.xlsx (14.09 kB)

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Table S6 junction p-values.xlsx (10.27 kB)

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Table S7 ade6 p-values.xlsx (10.44 kB)

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Supplemental Material for Joshi, Musicante, and Wheeler, 2021

figure

posted on 2021-05-26, 16:21 authored by Arati Joshi, Meryl J. Musicante, Bayly S. Wheeler

Supplemental Figures and Tables for Joshi et al.

Figure S1: The RCE flanking sequences are sufficient to establish heritable gene silencing. The extent of silencing was measured by plating RCE(∆RCcons)-ade6⁺and RCcons-ade6⁺ yeast on adenine-limiting media and counting the number of colonies with visible signs of silencing, including red, pink, and sectored phenotypes. Representative images, along with the percentage of colonies that exhibited silencing, are shown for three biological replicates of each genotype. For RCE(∆RCcons)-ade6⁺strains, colonies that exhibited silencing werecultured overnight and plated on adenine limiting media to measure the stability of silencing.