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Supplemental Material for Joshi, Musicante, and Wheeler, 2021
figure
posted on 2021-05-26, 16:21 authored by Arati Joshi, Meryl J. Musicante, Bayly S. WheelerSupplemental Figures and Tables for Joshi et al.
Figure S1: The RCE
flanking sequences are sufficient to establish heritable gene silencing. The extent of silencing was measured by plating RCE(∆RCcons)-ade6+
and RCcons-ade6+ yeast on
adenine-limiting media and counting the number of colonies with visible signs
of silencing, including red, pink, and sectored phenotypes. Representative
images, along with the percentage of colonies that exhibited silencing, are
shown for three biological replicates of each genotype. For RCE(∆RCcons)-ade6+
strains, colonies that exhibited silencing were cultured overnight
and plated on adenine limiting media to measure the stability of silencing.