Supplemental Material for Doll, Soltanmohammadi, and Schumacher, 2019

Supplemental information.pdf contains all Supplemental Figure Legends and descriptions of the Supplementary Tables

Fig S1 alg-2(ok304) mutants display numerous apoptotic corpses accumulating in the proximal germline 4 h post-IR.

Fig S2 Cytoplasmic ALG-2::HA expression in alg-2;palg-2::ALG-2::HA germlines.

Fig S3 alg-2 mutant animals exhibit mild HR-directed repair defect and a subtle delay in mitotic cell cycle arrest upon IR exposure.

Fig S4 EGL::V5 can trigger apoptosis in consequence to DNA damage.

Fig S5 SIR-2.1 partially contributes to DNA damage-induced apoptosis in alg-2 mutant worms.

Fig S6 MPK-1 is hyperphosphorylated in alg-2 mutant germline loops upon IR exposure.

Fig S7 Reduced ERK MAPK signaling partially rescues elevated apoptosis in alg-2(ok304) mutant animals after exposure to 90 Gy of γ-irradiation.

Fig S8 cep-1(gk138) deletion abolishes MPK-1 phosphorylation in IR-treated alg-2(ok304) mutant germline loops.

Fig S9 ced-9(n1950) gain of function abolishes MPK-1 phosphorylation in IR-treated alg-2(ok304) mutant germline loops.

Fig S10 Weak ced-3 mutant alleles block MPK-1 phosphorylation in the gonad loop of alg-2(ok304) mutant animals.

Fig S11 MPK-1 phosphorylation is suppressed in CED-1::GFP-positive late-pachytene germ cells undergoing apoptosis.

Fig S12 MPK-1 phosphorylation is abrogated in late apoptotic corpses in the alg-2(ok304) mutant germline.

Supplementary table 1: pdf-format: miRNA regulation wild type IR-treated vs wild type untreated

Supplementary table 2: pdf-format: miRNA regulation alg-2(ok304) untreated vs wild type untreated

Supplementary table 3: pdf-format: miRNA regulation alg-2(ok304) IR-treated vs alg-2(ok304) untreated

Supplementary table 4: pdf-format: miRNA regulation alg-2(ok304) IR-treated vs wild type IR-treated