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Supplemental Material for Doll, Soltanmohammadi, and Schumacher, 2019

figure
posted on 11.07.2019 by Markus Alexander Doll, Najmeh Soltanmohammadi, Björn Schumacher

Supplemental information.pdf contains all Supplemental Figure Legends and descriptions of the Supplementary Tables

Fig S1 alg-2(ok304) mutants display numerous apoptotic corpses accumulating in the proximal germline 4 h post-IR.

Fig S2 Cytoplasmic ALG-2::HA expression in alg-2;palg-2::ALG-2::HA germlines.

Fig S3 alg-2 mutant animals exhibit mild HR-directed repair defect and a subtle delay in mitotic cell cycle arrest upon IR exposure.

Fig S4 EGL::V5 can trigger apoptosis in consequence to DNA damage.

Fig S5 SIR-2.1 partially contributes to DNA damage-induced apoptosis in alg-2 mutant worms.

Fig S6 MPK-1 is hyperphosphorylated in alg-2 mutant germline loops upon IR exposure.

Fig S7 Reduced ERK MAPK signaling partially rescues elevated apoptosis in alg-2(ok304) mutant animals after exposure to 90 Gy of γ-irradiation.

Fig S8 cep-1(gk138) deletion abolishes MPK-1 phosphorylation in IR-treated alg-2(ok304) mutant germline loops.

Fig S9 ced-9(n1950) gain of function abolishes MPK-1 phosphorylation in IR-treated alg-2(ok304) mutant germline loops.

Fig S10 Weak ced-3 mutant alleles block MPK-1 phosphorylation in the gonad loop of alg-2(ok304) mutant animals.

Fig S11 MPK-1 phosphorylation is suppressed in CED-1::GFP-positive late-pachytene germ cells undergoing apoptosis.

Fig S12 MPK-1 phosphorylation is abrogated in late apoptotic corpses in the alg-2(ok304) mutant germline.

Supplementary table 1: pdf-format: miRNA regulation wild type IR-treated vs wild type untreated

Supplementary table 2: pdf-format: miRNA regulation alg-2(ok304) untreated vs wild type untreated

Supplementary table 3: pdf-format: miRNA regulation alg-2(ok304) IR-treated vs alg-2(ok304) untreated

Supplementary table 4: pdf-format: miRNA regulation alg-2(ok304) IR-treated vs wild type IR-treated

History

Article title

ALG-2/AGO-Dependent mir-35 Family Regulates DNA Damage-induced Apoptosis Through MPK-1/ERK MAPK Signaling Downstream of the Core Apoptotic Machinery in Caenorhabditis elegans

Manuscript #

GENETICS/2019/302458

Article DOI

10.1534/genetics.119.302458

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