Supplemental Material for Crain et al., 2022
figureposted on 05.04.2022, 14:15 by Aaron T. Crain, Stephen Klusza, Robin L. Armstrong, Priscila Santa Rosa, Brenda R. S. Temple, Brian D. Strahl, Daniel J. McKay, A. Gregory Matera, Robert J. Duronio
Structures after 500 ns simulations of all four replicates for Set8WT, Set8RG, and Set8RGHL from Figure 3B.
Raw western blot images for Set8 and β-Tubulin (Figure 4B,C). The first number of each replicate indicates the biological sample number and the decimal indicates the technical replicate number. Replicate 3.1 was not used in the quantification due to low Oregon-R control signal.
A) Raw western blot images for H4K20me1, pan H4, pan H3, and Fibrillarin (Figure 4D, E). The first number of each replicate indicates the biological sample number and the decimal indicates the technical replicate number. B) Quantification of anti-H4K20me1 signal on western blots by densitometry (see methods). Shown is the mean and standard deviation of measurements (circles) across three biological replicates (two for 1x Set8RG) normalized to pan H4 signal. Oregon-R normalized signal was set to 1 for each replicate. Significance was determined by a one-way Anova followed by Tukey’s multiple comparison test. ** indicates p<0.01, **** indicates p<0.0001, and ns indicates not significant. Data is the same as Figure 4E with the addition of two replicates of 1x Set8RG.