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Supplemental Material for Biernat et al., 2021

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posted on 2021-02-08, 16:56 authored by Emily Biernat, Jeena Kinney, Kyle Dunlap, Christian Rizza, Chhabi K. Govind
Figure S1 shows nucleosome profiles generated by MNase-seq, Pol II ChIP-seq profiles and correlation between biological replicates for all datasets. The histograms depicting the MPDF profiles from the two earlier studies (Kubik et al. 2018; Rawal et al. 2018) are also shown. Figure S2 shows RSC enrichment in transcribed CDSs genome-wide. Figure S3 shows 2-dimensional RSC MNase ChIP-seq profiles for replicates. Figure S4 shows genome-browser shots of Pol II and nucleosome occupancies in WT and Sth1 anchor-away cells (Kubik et al. 2018). Table S1 provides names and genotype of the strains used in this study.

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Article title

The RSC complex remodels nucleosomes in transcribed coding sequences and promotes transcription in Saccharomyces cerevisiae