posted on 2021-04-09, 18:32authored byAlec Barrett, Rebecca McWhirter, Seth R. Taylor, Alexis Weinreb, David M. Miller III, Marc Hammarlund
Supplementary material describing comparisons between two RNA-sequencing library preparation techniques for low input samples in C. elegans. Two techniques were compared head-to-head on the same samples, a PolyA selection based approach (SMARTseq V4, Takara), and a ribodepletion based approach (SoLo Ovation, Tecan Genomics) with a custom ribodepletion probe set designed for C. elegans.
Here we describe: correlation analyses within and between each kit; gene level variance measures compared between the two techniques for all protein coding genes; and gene level variance comparisons between both techniques for the 2000 shortest and longest protein coding genes.
We also provide supplementary tables describing: metrics for sample inputs and mapping rates for all samples; mean and median values of variance measures in 5 ranked groups for both techniques, and comparisons between sample preparation in this study and a previous study.
A head-to-head comparison of ribodepletion and polyA selection approaches for C. elegans low input RNA-sequencing libraries