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Supplementary Material for Wernick et al. 2019

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posted on 2019-02-05, 19:14 authored by Riana I. Wernick, Stephen F. Christy, Dana K. Howe, Jennifer A. Sullins, Joseph F. Ramirez, Maura Sare, McKenna J. Penley, Levi T. Morran, Dee R. Denver, Suzanne Estes
Supplementary figures show the following; S1 and S2: relative and competitive fitness of gas-1 RC lines, respectively; S3: normalized mtDNA copy number for all C. elegans lines; S4: expected and observed percentages of gas-1 RC line genic mtDNA mutations; S5: chromatograms depicting evolutionary trajectories of two mtDNA variants; S6: GO enrichments and interactome results for gas-1 RC line mutations; S7: GO term categorizations for gas-1 RC line SNPs.

Supplementary tables contain the following data; S1: Illumina HiSeq run statistics; S2: ANOVA results for C. elegans life-history traits; S3: results of mtDNA copy number normalization; S4: gas-1 G0 mutations reverted to wildtype alleles within RC lines; S5: mtDNA SNPs; S6: nDNA SNPs; S7: gas-1 RC line SNPs occurring within the gas-1 interactome.

File S1 shows the frequencies within each gas-1 RC line for all mtDNA positions in which a variant was identified.

File S2 shows the frequencies within each gas-1 MA line for the fox-1 single-basepair reversion discovered within three gas-1 RC lines.

File S3 contains custom code for simulations reported in Figure S5.

File S4 contains a complete accounting of gas-1 RC G0 enrichment results.


Article title

Sex and Mitonuclear Adaptation in Experimental Caenorhabditis elegans Populations

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