Supplemental Material for Xu at al., 2019.pdf (2.77 MB)
Supplemental Material for Xu et al., 2019
datasetposted on 2019-04-08, 13:26 authored by Xingya Xu, Norihiko Nakazawa, Li Wang, Orie Arakawa, Mitsuhiro Yanagida
Table S1 lists homologs of S. pombe centromeric proteins in human and S. cerevisiae. Figure S1 shows micrographs of exo2 and pan2 mutants. Figure S2 validates Mis17 protein bands detected by an anti-Mis17 antibody. Figure S3 shows Mis17 and Cnp1/spCENP-A protein levels in EKC/KEOPS complex mutants.Figure S4~S6 are sequence alignment of Bud32, Kae1 and Sua5, respectively. Figure S7 describes mRNA measurement of mis17 and mis6 genes in wild type and mutants.