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Supplemental Material for Xiao et al., 2019

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posted on 17.10.2019 by Chengfeng Xiao, Danna Hull, Shuang Qiu, Joanna Yeung, Jie Zheng, Taylor Barwell, R Meldrum Robertson, Laurent Seroude
Supplemental Figure S1 contains western-blot data obtained with an HSP70 antibody. Supplemental Figure S2 shows the outcome of the survival data to thermal, oxidative and metabolic stresses of 3 days and 10 days old females overexpressing HSP70. Supplemental Figure S3 shows the outcome of the longevity data of females overexpressing HSP70. Supplemental Figure S4 reports the cloning steps performed to obtain the pCX9 plasmid. Supplemental Figure S5 contains western-blot data that show the down-regulation of Hsp70 by the UAS-hsp70-RNAi transgenes. Supplemental Table S1 contains the data and statistical analysis of the survival during development. Supplemental Table S2 contains the data and statistical analysis of the stress resistance experiments. Supplemental Table S3 contains the data and statistical analysis of the longevity experiments with the UAS-hsp70 transgene. Supplemental Table S4 contains the data and statistical analysis of the longevity experiments with the UAS-hsp70-RNAi transgene. The reagent table provides the information and source of transgenic strains, DNA plasmids and antibodies used in this study.

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Article title

Expression of Heat Shock Protein 70 Is Insufficient To Extend Drosophila melanogaster Longevity

Manuscript #

G3/2019/400782

Article DOI

10.1534/g3.119.400782

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