Supplemental Material for Wang et al., 2020

Figure S1. Structure of the rsu-1 locus.

Figure S2. Depletion of RasLET-60 and RSU-1 suppresses cytoplasmic protrusions in rsu-1(tm6690).

Figure S3. Depletion of RasLET-60 suppresses the multivulva in let-60(n1046).

Figure S4. RSU-1 does not regulate subcellular localization of IntegrinPAT-3 and PINCHUNC-97.

Figure S5. Depletion of RSU-1 does not change the focal adhesion sites in vulval muscle.

Figure S6. The morphology of vm1 muscle cells in L4 stage and adult stage.

Table S1. C. elegans strains used in this study.

Table S2. Primers used in this study.

Table S3. The gene and clone of several actin bundles protein candidates.

Table S4. The sequence of mCherry::RSU-1.

Table S5. The sequence of GFP::UNC-97.