Supplemental Material for Wagner et al., 2025

Laboratory cultures of cyanobacteria often have other bacteria growing  with them that cannot be easily removed. When you isolate DNA from the  cyanobacterium to sequence its genome, you also get DNA from the other  bacteria too. As cyanobacteria are hard to extract DNA from, you  sometimes get more DNA from the other bacteria than from the  cyanobacterium. We realized that these problems might be able to solve  each other, by repeating our DNA extraction on the same cells of  cyanobacterium, we saw DNA from the other bacteria in the early  fractions, with cyanobacterial DNA enriched in the later fraction.