13 files

Supplemental Material for Telzrow et al., 2021

posted on 20.08.2021, 18:28 authored by Calla L. Telzrow, Paul J. Zwack, Shannon Esher Righi, Fred S. Dietrich, Cliburn Chan, Kouros Owzar, J. Andrew Alspaugh, Joshua A. Granek

Figure S1 contains a depth of coverage plot of the mitochondrial rRNA genes. Figures S2, S3, and S4 display scatterplot visualizations of rRNA depletion specificity summarized in Figures 2, 3, and 4, respectively. Figure S5 displays the rRNA depletion efficiency for ncRNA genes, excluding CNAG_12993. Figure S6 displays a scatterplot visualization of the genes that are underrepresented by the Poly(A) isolation method, Figure S7 displays the read depth across each of these genes, and Table S1 provides details of these genes. Figure S8 displays the read depth across each of the predicted lncRNA genes identified by LncPipe analysis. File S1 contains the RNase H depletion protocol. File S2 contains the Ensembl GTF with newly annotated mitochondrial rRNA. File S3 contains DNA oligonucleotide sequences used in the RNase H depletion method.


Article title

Comparative analysis of RNA enrichment methods for preparation of Cryptococcus neoformans RNA sequencing libraries