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Supplemental Material for Stevenson et al., 2020

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posted on 2020-08-19, 16:53 authored by Zachary C. Stevenson, Megan J. Moerdyk-Schauwecker, Brennen Jamison, Patrick C. Phillips

Figure S1: Schematic of landing pad insertion vector pMS74.

Figure S2: Schematic of cargo insertion vector pMS81

Figure S3: Comparison of integration broods.

Figure S4: Representative images of eft-3p::wrmScarlet::tbb-2 3’UTR (pZCS16) array individuals.

Figure S5: Injection of a Cre expressing plasmid results in the efficient removal of the hygromycin resistance marker gene.

Table S1. Strains generated for this study.

Table S2. Plasmids generated for this study.

Table S3. Primers used in this study.

File S1. Sequence file for PX696.

History

Article title

Rapid Self-Selecting and Clone-Free Integration of Transgenes into Engineered CRISPR Safe Harbor Locations in Caenorhabditis elegans

Manuscript #

G3/2020/401400-T

Article DOI

10.1534/g3.120.401400

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    G3: Genes|Genomes|Genetics

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