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Supplemental Material for Sorgini et al., 2021

dataset
posted on 30.06.2021, 14:44 by Crystal A. Sorgini, Lucas M. Roberts, madsen sullivan, Asaph B. Cousins, Ivan Baxter, Anthony J. Studer
Supplemental Tables S1-S5.
Table S1: Recombinant Inbred Lines used for single family QTL mapping of leaf δ13C, SLA, and ionomics.
Table S2: Recombinant Inbred Lines used for leaf δ13C joint linkage mapping.
Table S3: Lines used for leaf δ13C GWAS.
Table S4: Significant QTL identified in ionomics analyses.
Table S5: Weather data for field sites.

Supplemental Figure 1 NAM RIL Transgressive Segregation. NAM RIL families CML103 (A), CML333 (B), NC358 (C), and Tx303 (D) were sorted by δ13C and plotted. Parental lines are shown in red.

Supplemental Figure 2 Element and δ13C Full Correlation Matrix. A full correlation matrix of element and δ13C mean values is show. The diagonal displays histograms of each dataset. Pearson’s r is shown in the upper panel. Scatter plots and best fit line are shown in the lower panel.

Supplemental Figure 3 Element QTL Mapping by Chromosome. Significant element QTL are shown by maize chromosomes 1 through 10 on the x-axis (in cM). Each NAM RIL family is represented by a symbol; CML103 (○), CML333 (x), NC358 (□), and Tx303 (Δ). Each element is designated by color. Significance thresholds (dashed horizontal line) were determined using 200 permutations, alpha=0.05 for each QTL independently.

Supplemental Figure 4 Genome Wide Association Study for δ13C. Manhattan plot showing significance of SNPs derived from a mixed linear model using the Bayesian information criterion to select the optimal number of principal components. The significance threshold represents the Bonferroni correction of familywise error rate.

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Article title

The Genetic Architecture of Leaf Stable Carbon Isotope Composition in Zea mays and the Effect of Transpiration Efficiency on Leaf Elemental Accumulation