1/1
3 files

Supplemental Material for Siebert et al., 2022

dataset
posted on 23.08.2022, 14:43 authored by Amy E. Siebert, Jacob CorllJacob Corll, J. Paige Gronevelt, Laurel Levine, Linzi M. Hobbs, Catalina Kenney, Christopher L. E. Powell, Fabia U. Battistuzzi, Ruth Davenport, A. Mark Settles, W. Brad Barbazuk, Randal J. Westrick, Gerard J. Madlambayan, Shailesh Lal

This data set represents the results of RNA-seq transcriptome analysis for the submitted revised manuscript entitled:  Genetic Analysis of Human RNA Binding Motif Protein 48 (RBM48) Reveals an Essential Role in U12-Type Intron Splicing 


 

Dataset S1. Read counts and statistics for individual introns and exon-exon junctions

detected in RNA-seq experiments. Intron coordinates are given for the Homo_sapiens.GRCh38.87

reference genome annotation. Intron reads and exon-exon junction reads are summed by Vector Control

(VC) and Knockout (KO) K-562 sublines for all biological replicates. Introns were filtered for overlap with

the MIDB dataset (see RNA-seq Methods in main article). Prior to analysis, introns were filtered for a

minimum sum of 10 total exon-exon junction and intronic read counts with an intronic density (Di) > 0 in

both VC or KO populations.

Dataset S2. Read counts for individual introns and exon-exon junctions for each biological
replicate from Vector Control (VC) and Knockout (KO) K-562 sublines detected in RNA-seq experiments.

History

Article title

Genetic Analysis of Human RNA Binding Motif Protein 48 (RBM48) Reveals an Essential Role in U12-Type Intron Splicing