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Supplemental Material for Shirra et al., 2021

dataset
posted on 19.08.2021, 14:33 by Margaret K. Shirra, Rachel A. Kocik, Mitchell A. Ellison, Karen M. Arndt
Figure S1 is a western blot of histone H3 methylation levels in mutant yeast strains.

Figure S2 measures suppression of cryptic transcription by the rpd3 histone deacetylase mutation

Figure S3 measures FLO8 transcription in yeast strains by northern analysis and chromatin immunoprecipitation

Figure S4 measures effects of gcn5 mutation on cryptic initiation

Table S1 lists yeast strains

History

Article title

Opposing functions of the Hda1 complex and histone H2B mono-ubiquitylation in regulating cryptic transcription in Saccharomyces cerevisiae