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Supplemental Material for Salim et al., 2021

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posted on 11.03.2021, 14:51 by Devika Salim, William D. Bradford, Boris Rubinstein, Jennifer L. Gerton
Figure S1 contains additional validation of the qRIN assay. Figure S2 contains analysis of rDNA stability in fob1D mutants. Figure S3 contains analysis of the loss of MATALPHA-LEU2 in fob1D mutants. Figure S4 contains analysis of rDNA stability in nicotinamide. Figure S5 contains validation of the qRIN assay for the CUP1-MATa reporter strains. Figure S6 contains validation of hits with altered rDNA stability. Figure S7 contains functional validation and additional supporting information with regard to deletion of CUP2 in CUP1-MATa reporter strains. Figure S8 contains stress and locus-specific effects of copper treatment. Figure S9 contains data regarding how deletion of RTT109, HST3, and HST4 affect stability of TUB1. Figure S10 contains data regarding how H3K56 acetylation restricts transcription-induced amplification of the CUP1 array in a 17 copy strain. File S1 describes how to calculate repeat loss rates. File S2 describes the method for determining and the location of the MATa-LEU2 casette in reporter strains. Table S1 summarizes rDNA and CUP1 instability measurements from the literature. Table S2 is a list of yeast strains. Table S3 is a list of primers. Table S4 contains validation copy number measurements for 25S, CUP1, LEU2 and MATALPHA1 in various yeast strains. Table S5 contains the rDNA repeat loss rates and copy number measurements from the screens. Table S6 contains rDNA copy number measurements from the temperature sensitive mutant collection. Table S7 contains copy number measurements of subcultured CUP1-MATa strains.

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DNA replication, transcription, and H3K56 acetylation regulate copy number and stability at tandem repeats

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