Supplemental Material for Sae-Lee et al., 2020
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Figure S1. Representative images of degenerating HSN neurons. A-C, Degenerated HSN neurons had dim (arrow heads) or barely visible (dotted circle) cell bodies. D-G, Earlier evidence of HSN neuron degeneration was visible as blebbing of the cell body (arrows). H-I, Processes of degenerating HSN neurons also showed beading (H, arrows) that was not visible in healthy HSN neurons (I). Autofluorescence from intestine bright in panels H and I due to higher gain to visualize HSN axon. VC4 and VC5 neurons fluorescing out of focus in panels B-F.
Figure S2. APP::mCherry is expressed throughout the nervous system. A DIC and fluorescent photomicrographs at 40X along the length of the worm. A C-terminal mCherry tag shows APP expression. As expected, we found that APP expressed under a pan-neuronal promoter was seen throughout the entire nervous system including the nerve ring and along the ventral nerve cord in the absence (left) and presence (right) of exAPOE4. B Enlarged photomicrographs of the ventral nerve cord taken mid-body show that APP is expressed in a punctate pattern in the VCs both when APOE4 expression is absent (top) and present (bottom). C Enlarged photomicrographs show that APP is expressed in a punctate pattern in the HSNs.
Figure S3. APOE3 and APOE4 transgene levels. A, Semi-quantitative analysis of level of APOE transgene arrays in genomic DNA. Average band intensities of three independent genomic preparations from five worms/strain following PCR for human APOE presented as mean ± SEM. No significant differences were found when comparing levels for APOE3 vs APOE4 strains after correcting for multiple comparisons. B, Expression of APOE determined by RT-qPCR of mRNA isolated from 3 (integrated) or 9 (extrachromosomal) near-starved 6-cm plates of worms. DCt values reveal little difference in gene expression between strains. Values represent the mean ± SE of two independent biological replicates with three technical replicates each. Data is represented as gene expression relative to the reference control gene tba-1.
Table S1. List of strains used throughout our study arranged by Figure.