Supplemental Material for Redondo-Blanco and Langenhan, 2018
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Figure S1. (A-C) Plasmid maps and information of vectors generated in this study. (D) Step-by-step protocol for the design of primers to construct a gene-specific HDR vector based on pHD-attPCC-FRT-mW-FRT.
Figure S2. (A) Location of primers and main genetic elements on doubly modified chromosome for transgene insertion. (B) Exemplary genotyping result before phiC31-mediated integration of two transgenes into the same genome (2), after integration before (3) and after (4) recombinase-assisted marker removal from both engineered loci.