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Supplemental Material for Pletcher et al., 2019

dataset
posted on 29.04.2019 by Rose C. Pletcher, Sara L. Hardman, Sydney F. Intagliata, Rachael L. Lawson, Aumunique Page, Jason Tennessen
Figure S1 includes a schematic diagram of glycolysis and provides a summary of how the TRiP RNAi lines that target this pathway disrupt eye development. Figure S2 includes a schematic diagram of the TCA cycle and illustrates of how TRiP RNAi lines that target this pathway do not disrupt eye development. Table S1 provides the list of Drosophila genes involved in metabolism and nutrient sensing that were used to select the TRiP strains used in this study. Table S2 contains a list of the TRiP RNAi stocks used in this study and the eye phenotype associated with each strain. Table S3 contains the list of TRiP RNAi stocks that induced an eye phenotype during the initial screen (Table S3) and the phenotypes that were observed when these strains were reanalyzed. Table S4 contains the list of TRiP RNAi strains that were used to disrupt oxidative phosphorylation and the eye phenotype induced by each transgene. Table S5 contains the list of TRiP RNAi strains that were used to disrupt GPI anchor biosynthesis and the eye phenotype induced by each transgene. Table S6 contains the list of TRiP RNAi strains that were used to disrupt glycolysis and the eye phenotype induced by each transgene. Table S7 contains the list of TRiP RNAi strains that were used to disrupt the TCA cycle and the eye phenotype induced by each transgene. Table S8 contains the list of TRiP RNAi strains that were used to disrupt glutamate and glutamine metabolism and the eye phenotype induced by each transgene.

History

Article title

A Genetic Screen Using the Drosophila melanogaster TRiP RNAi Collection To Identify Metabolic Enzymes Required for Eye Development

Manuscript #

G3/2019/400193R1

Article DOI

10.1534/g3.119.400193

Licence

Exports