RNAi manuscript supplementary data_G3.xlsx (1.36 MB)

Supplemental Material for Parker et al., 2020

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posted on 22.01.2020 by Grace Parker, Nathan Kohn, Ally Spirina, Anna McMillen, Wen Huang, Trudy Mackay

Supplementary Table 1 gives the raw data for the four week productivity assay of the pure and reciprocal corsses between the O and B lines. Supplementary Table 2 gives the analyses of variance (ANOVAs) of O and B line productivity. Supplementary Table 3 shows the genomic regions containing genetically divergent SNPS. Supplementary Table 4 gives the list of 57 candidate genes tested, including their FlyBase ID, gene name, gene, symbol, and the Vienna stock number used in the RNAi assay. Supplementary Table 5 gives the raw lifespan data for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 6 gives the raw lifetime productivity data for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 7 gives the raw weekly productivity data for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 8 gives the analyses of variance (ANOVAs) of lifespan for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 9 gives the analyses of variance (ANOVAs) of lifetime productivity for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 10 gives the analyses of variance (ANOVAs) of weekly productivity for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 11 gives the analyses of variance (ANOVAs) of ovary qPCR for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 12 gives the analyses of variance (ANOVAs) of accessory gland qPCR for GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Table 13 is a summary of genes for which RNAi affects lifespan and/or productivity. Supplementary Figure 1 shows the results of ovary qPCR for the difference in expression between GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies. Supplementary Figure 2 show the results of accessory gland qPCR for the difference in expression between GAL4-c825 × UAS RNAi and GAL4-c825 × control F1 flies.

History

Article title

Genetic Basis of Increased Lifespan and Postponed Senescence in Drosophila melanogaster

Manuscript #

G3/2020/401041

Article DOI

10.1534/g3.120.401041

Licence

Exports