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Supplemental Material for Pallares, Picard, and Ayroles, 2020
dataset
posted on 2019-11-11, 20:22 authored by Luisa F. Pallares, Serge Picard, Julien F. AyrolesFile S1 contains step-by-step instructions for the TM3’seq library
preparation protocol. File S2 contains step-by-step instructions for the mRNA
extraction protocol. File S3 describes how to implement UMIs in the TM3’seq
standard protocol. Table S1 contains the sequences of the oligos used in the
TM3’seq protocol. Figure S1 describes general mapping parameters of the RNA samples
used in this manuscript. Figure S2 shows the effect of sequencing depth and
amplification cycles for TM3’seq libraries. Figure S3 summarized the results of
ERCC analysis of NEB and TM3’seq libraries. Figure S4 and S5 show the raw ERCC-Dashboard
output of the ERCC analysis for TM3'seq and NEB, respectively.