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Supplemental Material for Ohkuni et al., 2020

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posted on 28.02.2020 by Kentaro Ohkuni, Evelyn Suva, Wei-Chun Au, Robert L. Walker, Reuben Levy-Myers, Paul S. Meltzer, Richard E. Baker, Munira A. Basrai

Table S1. S. cerevisiae strains used in this study.

Table S2. Plasmids used in this study.

Table S3. Primers used in this study.

Figure S1. The stability of HA-Cse4 K215/216R/A is not increased when compared to HA-Cse4 in a psh1∆ strain.

Figure S2. Levels of chromatin associated Cse4 K215/216R/A are reduced when compared to wild-type Cse4.

Figure S3. Protein levels of Cse4 and Cse4 K215/216R under the copper-inducible promoter.

Figure S4. GAL-cse4 K65R exhibits SDL in slx5∆ and hir2∆ strains and those are suppressed by cse4 K215/216R.

History

Article title

Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-terminal Sumoylation

Manuscript #

GENETICS/2020/303090R1

Article DOI

10.1534/genetics.120.303090

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