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Supplemental Material for Ohkuni et al., 2020

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posted on 2020-02-28, 15:56 authored by Kentaro Ohkuni, Evelyn Suva, Wei-Chun Au, Robert L. Walker, Reuben Levy-Myers, Paul S. Meltzer, Richard E. Baker, Munira A. Basrai
<p><a></a><b>Table S1. </b><i>S. cerevisiae</i> strains used in this study.</p><p><b>Table S2.</b> Plasmids used in this study.</p><p><b>Table S3.</b> Primers used in this study.</p><p><b>Figure S1. </b>The stability of HA-Cse4 K215/216R/A is not increased when compared to HA-Cse4 in a <i>psh1</i>∆ strain. </p><p><b>Figure S2. </b>Levels of chromatin associated Cse4 K215/216R/A are reduced when compared to wild-type Cse4.</p><p><b>Figure S3. </b>Protein levels of Cse4 and Cse4 K215/216R under the copper-inducible promoter.</p><p><b>Figure S4. </b><i>GAL-cse4 K65R</i> exhibits SDL in <i>slx5</i>∆ and <i>hir2</i>∆ strains and those are suppressed by <i>cse4 K215/216R</i>.</p>

History

Article title

Deposition of Centromeric Histone H3 Variant CENP-A/Cse4 into Chromatin Is Facilitated by Its C-terminal Sumoylation

Manuscript #

GENETICS/2020/303090R1

Article DOI

10.1534/genetics.120.303090