Supplemental Material for Nonet, 2020

posted on 08.06.2020 by Michael L. Nonet

Figure S1. Extrachromosomal array behavior in presence of Flp.

Figure S2. Chromosomal location of RMCE landing sites.

Figure S3. Molecular Characterization of landing sites and insertions.

Figure S4. GFP fluorescence in various developmental stages of jsTi1493 animals.

Figure S5. Diagrams of reporter constructs.

Figure S6. Diagrams of driver constructs.

Figure S7. Kinetics of GFP expression in tet-OFF driver reporter lines.

Figure S8. Robust induction of tetO 7X promoter in doxycycline.

Figure S9. Comparison of background in the head region of RMCE reporter lines.

Figure S10. Comparison of background in the tail region of RMCE reporter lines.

Figure S11. Attempts at multi-plasmid insertion using RMCE.

Figure S12. Behavior of oligonucleotides and plasmids in the C. elegans germline.

Figure S13. Effects of DNA concentration on integration frequency.

Figure S14. Two step RMCE insertion at landing sites.

RMCE protocol. A PDF protocol of the RMCE method with overview and figures

Supplementary Methods. Describes details of all plasmid constructions.

Supplemental Figure Legends. Legends for the 14 Supplemental Figures.

Supplemental Statistics.

Table S1. Detailed summary of quantified injections.

Table S2. Other injections.

Table S3. DNA constructs.

Table S4. Oligonucleotides.

Table S5. Transgenes and alleles.

Table S6. Strains.

Table S7 Addgene plasmids.

Table S8 CGC strains.


Article title

Efficient Transgenesis in Caenorhabditis elegans Using Flp Recombinase-Mediated Cassette Exchange

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