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Supplemental Material for Nelson and Ambros, 2021

dataset
posted on 19.01.2021, 17:19 by Charles Nelson, Victor Ambros
Figure S1 contains sanger sequencing confirming let-7 microRNA sequence in C. macrosperma. Figure S2 contains an expanded view of the C. sulstoni and C. macrosperma genomic regions that are syntenic to the let-7 genomic region in C. elegans. Figure S3 contains the C. afra genomic regions that are syntenic to the let-7 genomic region in C. elegans. Figure S4 contains the developmental growth rates of C. elegans, C. macrosperma, and C. sulstoni. Figure S5 contains lin-4 microRNA expression throughout development in C. elegans, C. macrosperma, and C. sulstoni. Figure S6 contains a lin-41 gene tree in Caenorhabditis species. Figure S7 contains examples of the integration of lin-4 and let-7-family microRNAs in C. elegans and C. sulstoni. Figure S8 contains the predicted minimum free energy hybridizations of let-7-family microRNAs with complementary sites in the lin-41 mRNA 3' UTR in Caenorhabditis species. Table S1 contains let-7-family microRNA sequences in Caenorhabditis species. Table S2 contains homologs of heterochronic genes in C. elegans and C. sulstoni. Table S3 contains lin-41 3' UTR bulge-SM+SUP information in Caenorhabditis species. Table S4 contains lin-41 3' UTR GU-SM+SUP information in Caenorhabditis species. Table S5 contains a list of Caenorhabditis strains used in this study. Table S6 contains a list of oligos used in this study. Table S7 contains information on the C. sulstoni lin-41::GFP allele generated in this study. Table S8 contains the normalized reads used for Figure 3. Table S9 contains the exact timing of larval molts used for Figure S4.

History

Article title

A cohort of Caenorhabditis species lacking the highly conserved let-7 microRNA

Manuscript #

G3/2020/401944R1