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Supplemental Material for Nageshan, Krogan, and Cooper, 2024

posted on 2024-04-05, 13:20 authored by Rishi Kumar Nageshan, Nevan Krogan, Julia Promisel Cooper

Supplementary information:

Table S1: Unprocessed list of the genes in the top 25th percentile of strength of interaction with the indicated query strains, and corresponding NGR values. Note that NGR value of a target gene represents the effect of that gene deletion on growth at 19°C relative to 30°C in the query mutant background (see methods). Sheet 1 shows a list of positive genetic interactors with taz1D, while Sheets 2-5 show negative genetic interactors with the indicated query mutant.

Table S2: List of genes that show negative genetic interaction specifically with taz1D at 30°C. The first sheet shows gene information along with respective NG and Log2 NG values (see Methods). Sheet 2 shows the GO tags for the genes from Sheet 1. Sheet 3 is a summary of Sheet 2, indicating the number of genes corresponding to each GO tag. In addition, Sheet 4 in Table S2 provides a description of the GO analysis column headings of the table represented in Sheet 2.

Table S3: List of top 25th percentile suppressors of taz1D c/s. The sheet format follows that of Table S2, except that NGR and Log2NGR are presented (see Methods). The high confidence hits determined by the 2-standard deviation method are highlighted in purple in Sheet 1. GO tags and GO analysis summaries for the high confidence hits are presented in Sheets 2 and 3, respectively.

Table S4: List of genes whose deletion reverts the ability of rif1+ deletion to suppress taz1D c/s. The sheet format follows that of Table 2, with NGR values. These genes were identified as explained in Figure 3a and b.


Article title

Parallel genetic screens identify nuclear envelope homeostasis as a key determinant of telomere entanglement resolution in fission yeast

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