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Supplemental Material for Mok et al., 2020

dataset
posted on 10.09.2020 by Calvin Mok, Gabriella Belmarez, Mark L. Edgley, Donald Moerman, Robert Waterston
Supplemental figures, tables, and data accompanying Mok et al. manuscript "PhenoMIP: High-Throughput Phenotyping of Diverse C. elegans Populations via Molecular Inversion Probes"

Figure S1 contains abundance and read depth data for MIP library sequencing data from false positive datasets.

Figure S2 contains an analysis of read depth data for probe representation and read depth distribution from false positive datasets.

Figure S3 contains principal component analysis of pool M11 replicates after subdividing into sets by food source.

Figure S4 contains principal component analysis of pool M11 replicates after subdividing into sets by generation.

Figure S5 contains violin plots of mean FCR across all strains, separated by fitness class.

Figure S6 contains fold-change analysis by food source from MMP strain data.

Figure S7 contains an analysis of mean FCR for strains across all datasets and of control strain (VC20019) mean FCR across multiple experiments.

Figure S8 contains MIP-MAP data for class 0 and class 1 strains.

File SD1 contains sequence information for all MIPs designed for 2007 MMP strains and 40 wild isolates of C. elegans.

File SD2 contains MIP abundance and read data used in the analysis of false positive sequencing data.

File SD3 contains the mean fold-change rate data for all MMP strains across pooling experiments presented in this manuscript.

File SD4 contains mean fold-change rate data for all replicates of the wild isolate strains across the RNAi experiments as well as variant data for ED3052 mapped interval associated with emb-27 RNAi suppression.

File SD5 contains all sequencing read counts and strain mean abundance data derived from MIP analysis for the pooling experiments presented within the manuscript.

History

Article title

PhenoMIP: High-Throughput Phenotyping of Diverse Caenorhabditis elegans Populations via Molecular Inversion Probes

Manuscript #

G3/2020/401656-T

Article DOI

10.1534/g3.120.401656

Licence

Exports