Supplemental Material for McClanahan et al., 2020
datasetposted on 08.10.2020 by Patrick D. McClanahan, Richard J. McCloskey, Melanie Ng Tung Hing, David M. Raizen, Christopher Fang-Yen
Datasets usually provide raw data for analysis. This raw data often comes in spreadsheet form, but can be any collection of data, on which analysis can be performed.
Supplemental Video File S1: Time lapse video of dehydration, rehydration, and recovery of C. elegans. A 10 µL droplet of N2 worms in M9 buffer containing 15% trehalose was pipetted on a PDMS surface and dehydrated for 12 h. The dehydrated droplet was transferred to a seeded NGM plate for recovery.
Supplemental Protocol File S2: Detailed protocol for dehydration, freezing, rehydration, and recovery of C. elegans.