Supplemental Material for McClanahan et al., 2020
datasetposted on 2020-10-08, 17:34 authored by Patrick D. McClanahan, Richard J. McCloskey, Melanie Ng Tung Hing, David M. Raizen, Christopher Fang-Yen
Supplemental Video File S1: Time lapse video of dehydration, rehydration, and recovery of C. elegans. A 10 µL droplet of N2 worms in M9 buffer containing 15% trehalose was pipetted on a PDMS surface and dehydrated for 12 h. The dehydrated droplet was transferred to a seeded NGM plate for recovery.
Supplemental Protocol File S2: Detailed protocol for dehydration, freezing, rehydration, and recovery of C. elegans.