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Supplemental Material for McClanahan et al., 2020

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posted on 08.10.2020 by Patrick D. McClanahan, Richard J. McCloskey, Melanie Ng Tung Hing, David M. Raizen, Christopher Fang-Yen
Supplemental Video File S1: Time lapse video of dehydration, rehydration, and recovery of C. elegans. A 10 µL droplet of N2 worms in M9 buffer containing 15% trehalose was pipetted on a PDMS surface and dehydrated for 12 h. The dehydrated droplet was transferred to a seeded NGM plate for recovery.

Supplemental Protocol File S2: Detailed protocol for dehydration, freezing, rehydration, and recovery of C. elegans.

History

Article title

Dehydrated Caenorhabditis elegans Stocks are Resistant to Multiple Freeze-Thaw Cycles

Manuscript #

G3/2020/401825

Article DOI

10.1534/g3.120.401825

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