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Supplemental Material for Maheshwari et al., 2021

dataset
posted on 2021-07-23, 16:32 authored by Richa Maheshwari, Mohammad M. Rahman, Daphna Joseph-Strauss, Orna Cohen-Fix

Supplemental Image Collections S1-S9 provide confocal microscopy images of C. elegans embryos expressing the ER marker SP12 fused to GFP (in green) and histone H2B fused to mCherry (in red), following treatment with RNAi against the specified genes. Related to Figures 6 and 7.

Supplemental Table S1 describes the nuclear morphology phenotypes caused by down-regulation of the indicated genes by RNAi in C. elegans.

Supplemental Table S2 provides the number of shared instances between the different phenotypic categories shown graphically in Figure 2B.

Supplemental Tables S3-S5 provide the raw data of the statistical overrepresentation analysis of Gene Ontology (GO) terms using the PANTHER Classification System based on Biological Process (B), Cellular Component (C), and Molecular Function classes (D), shown graphically in Figure 3B-D.

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Article title

An RNAi screen for genes that affect nuclear morphology in Caenorhabditis elegans reveals the involvement of unexpected processes