Supplemental Material for Ma et al., 2019

The Neurospora crassa wild type strans were growthed in minimal slants for two weeks and cultivated in 2% glucose media under DD condition. The strain was cultured under dark for 12h and 20h. And RNA libraries were prepared for sequencing using standard Illumina protocols after total RNA was extracted. Then RNA-seq was performed. Differential expression analysis of two samples was performed using the previous statistics model. The resulting P-values were adjusted using the Benjamini and Hochberg’s approach for controlling the false discovery rate and adjusted P-value <0.05 were assigned as differentially expressed gene.