Version 2 2020-07-28, 14:02Version 2 2020-07-28, 14:02
Version 1 2020-07-28, 13:58Version 1 2020-07-28, 13:58
dataset
posted on 2020-07-28, 14:02authored byJian Liu, Laureline Mosser, Catherine Botanch, Jean-Marie François, Jean-Pascal Capp
Figure
S1 shows the sequence of the synthetized tdTomato gene with flanking homologies
to SIR2. Figure S2 illustrates the growth of clones with subtelomeric URA3
either on –ura plates or on 5-FOA plates. Figure S3 and S4 describe the
recovery of the initial expression profile after cell sorting for both low and
high Sir2-expressing cells, after 6 h and 24 h respectively. Figure S5 shows
the viability of subpopulations of the tdTomato-Tsl1-tagged strain expressing
extreme tdTomato levels similar than in the tdTomato-Sir2-tagged strain. Table
S1 lists the primers used in this study. Table S2 contains the raw data of the
silencing and viability analyses
History
Article title
SIR2 Expression Noise Can Generate Heterogeneity in Viability but Does Not Affect Cell-to-Cell Epigenetic Silencing of Subtelomeric URA3 in Yeast