1/1
0/0

Supplemental Material for Lange, Suleman, and Srayko, M., 2019

dataset
posted on 24.04.2019 by Karen Lange, Aly Suleman, Martin Srayko
p.p1 {margin: 0.0px 0.0px 0.0px 0.0px; font: 11.0px Arial}

Figure S1 shows anti-Ska immunofluorescence in one-cell embryos treated with control or ska-1 RNAi. Figure S2 shows KLP-7-GFP in the one-cell embryo is not altered by ska-1(RNAi) or ska-3(RNAi). Figure S3 shows Ska mutations do not restore microtubule levels in rsa-1(or598) embryos. Figure S4 shows TPXL-1-GFP at the centrosome is not altered by ska-1(RNAi). Figure S5 shows Ska is required to form a tight metaphase plate. Figure S6 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of Ska function. Figure S7 shows the rsa-1(or598) spindle-collapse phenotype is partially rescued by loss of KMN kinetochore components and loss of EBP-2. Table S1 contains information on C. elegans strains used in this study. Table S2 contains information on cloning primers used in this study.

History

Article title

Kinetochore Recruitment of the Spindle and Kinetochore-Associated (Ska) Complex is Regulated by Centrosomal PP2A in Caenorhabditis elegans

Manuscript #

GENETICS/2019/302105

Article DOI

10.1534/genetics.119.302105

Licence

Exports