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Supplemental Material for Kweon et al., 2018

dataset
posted on 03.05.2018 by Sin Ho Kweon, Jongbin Lee, Chunghun Lim, Joonho Choe

Figure S1 sraKO mutant flies display normal morphology in axonal projections from PDF-expressing circadian pacemaker neurons.

Figure S2 sraKO mutation decreases PER and TIM levels in PDF-expressing circadian pacemaker neurons. Adult fly brains were dissected at ZT23 in LD cycles.

Figure S3 A transgenic reporter for sra promoter activity (sra-Gal4) is expressed in various brain regions of adult fly.

Figure S4 sra depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.

Figure S5 Conditional depletion of sra in PDF-expressing neurons causes poor rhythmicity in free-running locomotor behaviors.

Figure S6 Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors.

Figure S7 Pharmacological inhibition of calcineurin reduces the steady-state levels of PER and TIM, but not CLK proteins, in Drosophila S2 cells.

Table S1 sra mutant flies display poor rhythmicity in free-running locomotor behaviors in DD.

Table S2 sra depletion in PDF-expressing clock neurons is sufficient to dampen rhythm amplitude in free-running circadian locomotor behaviors.

Table S3 Conditional depletion of sra in PDF-expressing neurons dampens free-running circadian locomotor rhythms.

Table S4 sra overexpression in circadian pacemaker neurons disrupts free-running locomotor rhythms in DD.

Table S5 Genomic deletion of each calcineurin subunit weakens rhythm amplitude in free-running circadian locomotor behaviors.

Table S6 A constitutively active form of Pp2B-14D disrupts free-running circadian behaviors in a dose-dependent manner.

Table S7 sra genetically suppresses Pp2B-14DACT to sustain circadian locomotor rhythms.

Table S8 Oral administration of cyclosporine A (CsA) lengthens circadian periods in free-running locomotor rhythms of wild-type flies.

History

Article title

High-Amplitude Circadian Rhythms in Drosophila Driven by Calcineurin-Mediated Post-translational Control of sarah

Manuscript #

GENETICS/2018/300808R1

Article DOI

10.1534/genetics.118.300808

Licence

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