Supplemental Material for Iwata et al., 2021

All genotyping primer data can be found in Supplemental Table 1 and gRNA data can be found in Supplemental Table 2. Figure S1 shows PCR amplification of the inversion In(6)1J break points in (Tprkb^em1Cu/In(6)1J × Tprkb^em1Cu/In(6)1J) F2 mice. Figure S2 shows the N-ethyl-N-nitrosourea mutagenesis scheme used to cross B6.C3H-In(6)1J Mitf^em1Cu strains and isolate recessive mutants.