Iwata et al_Supplementary_.pdf (1.6 MB)
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Supplemental Material for Iwata et al., 2021

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posted on 01.06.2021, 18:36 authored by Satoru Iwata, Takahisa Sasaki, Miki Nagahara, Takashi Iwamoto
All genotyping primer data can be found in Supplemental Table 1 and gRNA data can be found in Supplemental Table 2. Figure S1 shows PCR amplification of the inversion In(6)1J break points in (Tprkbem1Cu/In(6)1J × Tprkbem1Cu/In(6)1J) F2 mice. Figure S2 shows the N-ethyl-N-nitrosourea mutagenesis scheme used to cross B6.C3H-In(6)1J Mitfem1Cu strains and isolate recessive mutants.


Article title

An efficient i-GONAD method for creating and maintaining lethal mutant mice using an inversion balancer identified from the C3H/HeJJcl strain